Fig. 2: Downregulation of circZNF609 impairs HCC cell proliferation, metastasis, and stemness. | Cell Death & Disease

Fig. 2: Downregulation of circZNF609 impairs HCC cell proliferation, metastasis, and stemness.

From: CircZNF609 enhances hepatocellular carcinoma cell proliferation, metastasis, and stemness by activating the Hedgehog pathway through the regulation of miR-15a-5p/15b-5p and GLI2 expressions

Fig. 2

a The knockdown efficiency of circZNF609 was measured by RT-qPCR in HCCLM3 and MHCC-97H cells. b, c CCK-8 and colony formation assays were carried out to measure the proliferation ability of transfected cells. d Sphere formation assay was used to evaluate cell sphere formation ability in HCCLM3 and MHCC-97H cells transfected with different plasmids. e–g The apoptosis capability of transfected cells was analyzed by flow cytometry, TUNEL, and western blot analyses. h Transwell assay was performed to examine the migration and invasion abilities of transfected cells. I Western blot analysis depicted that knocking down circZNF609 could suppress cell metastasis, EMT, and stemness. **P < 0.01.

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