Fig. 4: CircZNF609 activates Hedgehog pathway via the regulation of miR-15a-5p/15b-5p and GLI2 expressions in HCC. | Cell Death & Disease

Fig. 4: CircZNF609 activates Hedgehog pathway via the regulation of miR-15a-5p/15b-5p and GLI2 expressions in HCC.

From: CircZNF609 enhances hepatocellular carcinoma cell proliferation, metastasis, and stemness by activating the Hedgehog pathway through the regulation of miR-15a-5p/15b-5p and GLI2 expressions

Fig. 4

a Western blot analysis was applied to detect the expression of GLI1, GLI2, GLI3, and GLI4 in different groups. b Subcellular fractionation and FISH assays were adopted to detect the distribution of circZNF609 in cytoplasm and nucleus. c StarBase was utilized and obtained seven miRNAs that not only could bind with circZNF609, but also could bind with GLI2. Moreover, then RT-qPCR was performed to detect the expression of these miRNAs in HCC cell lines and normal human liver epithelial cell line. d, e The expression of miR-15a-5p/15b-5p and GLI2 was detected in HCC tissues and peri-tumor. f, g The correlation between miR-15a-5p/15b-5p and circZNF609 (or GLI2), together with the correlation between circZNF609 and GLI2 was analyzed by Spearman’s correlation analysis. *P < 0.05, **P < 0.01.

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