Fig. 5: CircZNF609 mediates HCC progression through the regulation of miR-15a-5p/15b-5p and GLI2 expressions. | Cell Death & Disease

Fig. 5: CircZNF609 mediates HCC progression through the regulation of miR-15a-5p/15b-5p and GLI2 expressions.

From: CircZNF609 enhances hepatocellular carcinoma cell proliferation, metastasis, and stemness by activating the Hedgehog pathway through the regulation of miR-15a-5p/15b-5p and GLI2 expressions

Fig. 5

a–c The relationship among circZNF609, miR-15a-5p/15b-5p, and GLI2 was confirmed by RIP, RNA pull-down, and luciferase reporter assays. d, e The proliferation ability of transfected cells was measured by CCK-8 and colony formation assays. f Cell sphere formation ability in transfected cells was examined by sphere formation assay. g, h Flow cytometry, TUNEL, and western blot analyses were conducted to evaluate the apoptosis capability of transfected cells. i Transwell assay was utilized to examine cell migration and invasion capabilities in transfected cells. j Cell metastasis, EMT, and stemness in different groups were examined by western blot analysis. *P < 0.05, **P < 0.01, ***P < 0.001.

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