Fig. 4: CircGCN1L1 interacts with miR-330-3p and is involved in the regulation of synoviocyte proliferation and chondrocyte apoptosis. | Cell Death & Disease

Fig. 4: CircGCN1L1 interacts with miR-330-3p and is involved in the regulation of synoviocyte proliferation and chondrocyte apoptosis.

From: CircGCN1L1 promotes synoviocyte proliferation and chondrocyte apoptosis by targeting miR-330-3p and TNF-α in TMJ osteoarthritis

Fig. 4

a, b Human synoviocytes from the control patient were transfected with circGCN1L1 plasmid or miR-330-3p inhibitor. The proportion of cells in the G1, S, or G2 phase was detected using quantitative FACS analysis. N = 6 (two different samples for three independent experiments). *p < 0.05. c Edu cell proliferation assay on single cultured synoviocytes from the control patient and co-cultured chondrocyte. Synoviocytes were divided into different groups according to the transfection (circGCN1L1, circGCN1L1 control, shcircGCN1L1, shcircGCN1L1 control, shcircGCN1L1 and miR-330-3p, circGCN1L1 and miR-330-3p inhibitor, and blank control). Edu-labeled proliferating cells (green), DAPI-labeled nuclei (blue). N = 3 (one sample for three independent experiments). Scale bar, 500 μm. d TMJ synoviocytes from the control patient were transfected with one of various concentrations of plasmids and then co-cultured with condylar chondrocytes. Chondrocyte apoptosis was detected with Annexin V-FITC/PI double staining using quantitative FACS analysis. *p < 0.05. Data are presented as mean ± S.D. One-way ANOVA with Bonferroni test (c–f) was performed. FACS fluorescence-activated cell sorting, PI propidium iodide, FITC fluorescein isothiocyanate, DAPI dihydrochloride, Edu 5-ethynyl-2′-deoxyuridine, TMJ temporomandibular joint.

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