Fig. 2: NANOG-knockdown inhibits EMT in CD44(+) cells from HNSCC cell lines.

a Invasion and b migration assays of CD44(+) cells transduced with sh.NANOG or sh.Scr. c Western blotting of Nanog, CD44, and EMT-related proteins, N-cadherin, Snail, Slug, and Zeb1 in CD44(+) cells transduced with sh.NANOG or sh.Scr. d Immunostaining of CD44(+) cells transduced with sh.NANOG or sh.Scr for N-cadherin and Snail. e Colony formation of CD44(+) cells following transduction sh.NANOG or sh.Scr. Scale bar = 20 µm. Error bars represent standard deviation. *p < 0.05.