Fig. 6: Inhibition of the ERK1/2-Nanog signaling pathway inhibits EMT, lung metastasis, and infiltrative behavior of CD44(+) cells in SCC-25 human cell line. | Cell Death & Disease

Fig. 6: Inhibition of the ERK1/2-Nanog signaling pathway inhibits EMT, lung metastasis, and infiltrative behavior of CD44(+) cells in SCC-25 human cell line.

From: RETRACTED ARTICLE: ERK1/2-Nanog signaling pathway enhances CD44(+) cancer stem-like cell phenotypes and epithelial-to-mesenchymal transition in head and neck squamous cell carcinomas

Fig. 6

a Photos of immunodeficient mice injected with 5000 CD44(+) SCC-25 cells stably transduced with sh.Scr, or sh.ERK1/2. b Hematoxylin and Eosin (H&E) and IF staining of infiltrating cells for DAPI (blue), CD44 (green), and Snail (red). Dashed line indicates tumor border. The graph shows the number of CD44+/Snail+ infiltrating cells. c Representative images and quantitation of detectable lung nodules on the surface of whole lungs. d Lung metastasis from immunodeficient mice following H&E and IF staining for DAPI (blue), CD44 (red), and Nanog (green). e Representative images of patient tumor tissues following IF staining for DAPI (blue), CD44 (green), Nanog (red), p-ERK1/2 (yellow), and Snail (white). Error bars represent standard deviation. *p < 0.05.

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