Fig. 4: EOF2 induce neuronal differentiation via PKCθ activation without affecting glia formation in NPC cultures. | Cell Death & Disease

Fig. 4: EOF2 induce neuronal differentiation via PKCθ activation without affecting glia formation in NPC cultures.

From: A novel PKC activating molecule promotes neuroblast differentiation and delivery of newborn neurons in brain injuries

Fig. 4

a Relative expression of mRNA of the different PKC isozymes under differentiation conditions. mRNA quantification was performed by reverse transcription and real time qPCR and using the Δct method. The mRNA for PKC were measured and normalized to the levels of 18S rRNA. Data are means ± S.E.M. of five independent measurements. b Representative fluorescence microphotographs of neurosphere-derived adhered cells transfected with PKCθ siRNA, a combination of PKCθ siRNA and EOF2 or either mock (control). Neuronal cells were identified by the immunocytochemical detection of β-III-tubulin (red); glial cells are identified by the immunocytochemical detection of GFAP (green) and total nuclei were counterstained with DAPI (blue). Scale bar = 50 µm. c Graph represents the percentage of total cells (detected by DAPI nuclear staining) that were positive for β-III-tubulin expression after treatments expressed as the percentage of control. Data are the means ± S.E.M. of nine independent measurements (n = 9). Statistical analysis: two tailed unpaired Student’s t test of each condition compared with control (*p = 0.003 and #p = 0.029). d Graph represents the percentage of total cells (detected by DAPI nuclear staining) that were positive for GFAP expression after treatment expressed as the percentage of the control. Data are the means ± S.E.M. of nine independent measurements (n = 9).

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