Fig. 2: CaN-mediated dephosphorylation of Drp1 at S637 in glaucomatous retina. | Cell Death & Disease

Fig. 2: CaN-mediated dephosphorylation of Drp1 at S637 in glaucomatous retina.

From: Loss of AKAP1 triggers Drp1 dephosphorylation-mediated mitochondrial fission and loss in retinal ganglion cells

Fig. 2

a Western blot analyses for CaN in the retinas of 10-month-old glaucomatous DBA/2J and age-matched D2-Gpnmb+ mice. b Representative images from immunohistochemical analyses for CaN (green, arrowheads) co-labeled with RBPMS (red, arrowheads) in RGCs. Note that glaucomatous RGCs showed increases in CaN protein expression. Blue color indicates nucleus. c Quantitative analysis for fluorescent intensity showed a significant increase in CaN immunoreactivity in the retina of glaucomatous DBA/2J mice. d Western blot analyses for total Drp1 and phospho-Drp1 Ser637 in the retinas of glaucomatous DBA/2J and age-matched D2-Gpnmb+ mice. e Representative images from immunohistochemical analyses for total Drp1 (green, arrowheads) co-labeled with TUJ1 (red, arrowheads) in RGCs. Note that glaucomatous RGCs showed increases in total Drp1 protein expression. Blue color indicates nucleus. f Quantitative analysis for fluorescent intensity showed a significant increase in Drp1 immunoreactivity in the retina of glaucomatous DBA/2J mice. GCL, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; OPL, outer plexiform layer; ONL, outer nuclear layer. Mean ± SD; n = 3 (a, d) and n = 10 (c, f); **P < 0.01 and ***P < 0.001 (two-tailed unpaired Student’s t test). Scale bar: 20 μm.

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