Fig. 1: Activation of SK channels preserves cell survival in conditions of erastin-induced ferroptosis. | Cell Death & Disease

Fig. 1: Activation of SK channels preserves cell survival in conditions of erastin-induced ferroptosis.

From: SK channel-mediated metabolic escape to glycolysis inhibits ferroptosis and supports stress resistance in C. elegans

Fig. 1

HT22 cells treated with erastin (1.5 µM, 16 h) in the presence or absence of CyPPA (10 µM or 50 µM) in glucose medium. Cell viability was measured by an a MTT assay and b Annexin V/PI fluorescence by FACS and c xCELLigence system. CyPPA treatment is represented as grey curves. Mitochondrial function was assessed by FACS analysis of HT22 cells challenged with erastin (1.5 µM, 16 h) in the presence or absence of CyPPA (10 µM) in glucose. d Mitochondrial superoxide levels using MitoSOX and e MMP loss using TMRE were determined by FACS analysis. Data are presented as mean ± SD, n = 3–6, *p < 0.05, **p < 0.01, ***p < 0.001, #p < 0.05, ##p < 0.01, ###p < 0.001, *compared to control #compared to erastin alone. All experiments were independently repeated at least three times.

Back to article page