Fig. 2: Ferroptotic cell death is prevented by SK channel activation in galactose medium. | Cell Death & Disease

Fig. 2: Ferroptotic cell death is prevented by SK channel activation in galactose medium.

From: SK channel-mediated metabolic escape to glycolysis inhibits ferroptosis and supports stress resistance in C. elegans

Fig. 2

HT22 cells treated with erastin (1.5 µM, 16 h) in the presence or absence of CyPPA (10 µM or 50 µM) in galactose medium. Cell survival is shown by a MTT assay, b FACS analysis of Annexin V/PI, and c xCELLigence system. Mitochondrial function was assessed by FACS analysis of HT22 cells challenged with erastin (1.5 µM, 16 h) in the presence or absence of CyPPA (10 µM) in glucose. d Mitochondrial superoxide levels using MitoSOX and e MMP loss using TMRE were determined by FACS analysis. Data are presented as mean ± SD, n = 3–6, *p < 0.05, **p < 0.01, ***p < 0.001, #p < 0.05, ##p < 0.01, ###p < 0.001, *compared to control #compared to erastin alone. All experiments were independently repeated at least three times.

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