Fig. 3: The effects of SK channel activation on mitochondrial morphology.

a, b Representative panel of microscopy images of different conditions and timepoints (TMRM in white, Calcein in green and Hoechst in blue). c, d Mitochondrial “morphofunction” parameters evaluated by high-resolution microscopy are shown. Aspect ratio, cell mito ratio and roundness are descriptors extracted from TMRM. Integration of cellular and nuclear descriptors allows calculation of various ‘derived descriptors’ of which ‘norm cyto area’ is depicted in the lower right panel. Panels in c represent parameters after 24 h of CyPPA (1 µM) treatments, in d after 48 h of treatment in glucose (blue dots) or galactose (pink dots). Data are presented as mean ± SD, n = 3, ***p < 0.001 versus glucose alone, ^##p < 0.01, ^###p < 0.001 versus galactose only-treated cells. All experiments were independently repeated at least three times.