Fig. 5: ROS initiates CAP-induced pyroptosis. | Cell Death & Disease

Fig. 5: ROS initiates CAP-induced pyroptosis.

From: Cold atmospheric plasma induces GSDME-dependent pyroptotic signaling pathway via ROS generation in tumor cells

Fig. 5

a–c ROS in PC9 cells was detected after CAP exposure in the presence or absence of NAC (5 mM). a Representative bright-field and fluorescent images. b Representative flow cytometry histogram. c Qualitative flow cytometric analysis. d The cell viability was measured at 24 h after CAP treatment in the presence or absence of NAC. e–g The features of cell pyroptosis were determined at 24 h after CAP treatment in the presence or absence of NAC (5 mM). e Representative bright-field microscopic images in which red arrowheads indicated large bubbles emerging from the plasma membrane. Scale bar, 25 µm. f Apoptosis- and pyroptosis-related proteins as indicated detected by western blotting. g Release of LDH in culture supernatants. h CAP-induced ROS production was evaluated after zVAD treatment or knockdown of GSDME. i Phosphorylation of JNK and release of cytochrome c from mitochondria were detected by western blotting. All the data are presented as the mean ± SD from three independent experiments. *p < 0.05, **p < 0.01 and NS means no significant difference.

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