Fig. 2: Loss of BECN1 promotes cancer metastasis in CRC. | Cell Death & Disease

Fig. 2: Loss of BECN1 promotes cancer metastasis in CRC.

From: The autophagy-independent role of BECN1 in colorectal cancer metastasis through regulating STAT3 signaling pathway activation

Fig. 2

a LoVo and HCT116 cells stably expressing negative control shRNA, shRNA-BECN1#1 or shRNA-BECN1#2, as indicated, were examined by western blotting. The GAPDH protein was used as a loading control. b The viability of each cell line as indicated was examined by CCK8 assay in LoVo and HCT116 cells. c The colony-formation assay was used to evaluate cell growth in LoVo and HCT116 cells. d, e Knockdown of BECN1 significantly enhanced migration and invasion abilities, as determined by transwell assays in vitro, and the right panel shows the histograms of the results. Scale bar: 100 μm. f Wound-healing assays were performed in both HCT116 and LoVo cells. The right panels show the histograms of the results. The bars indicate the SD. *p < 0.05, **p < 0.01 using Student’s t-test. Scale bar: 200 μm.

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