Fig. 4: Berberine interfered with the interaction between human intestinal stromal cells and immune cells, in which OSMR functioned as an indispensible factor.

a Human CCD-18Co cells were transfected with NC or si-OSMR for 72 h and then stimulated with OSM (10 ng/ml) for 1 h in the presence of berberine (50 μM). The cells were collected for western blot assay (a) and immunofluorescent staining with p-STAT1 (b). c Calcein AM-labeled U937, THP-1, and Jurkat T cells were incubated on OSM-stimulated transfected CCD-18Co cells for 30 min. Adhered immune cells were visualized under Olympus IX73 microscope. d Representative images of Calcein AM-labeled U937, THP-1, and Jurkat T cells migrated to the supernatants from OSM-stimulated transfected CCD-18Co cells for 2 h. e The counting numbers of U937, THP-1, and Jurkat T cells chemotactic to the lower chamber containing OSM-stimulated transfected CCD-18Co cells supernatants. f The mRNA expression of certain chemokines in OSM-stimulated transfected CCD-18Co cells. g Protein level of CXCL10 in OSM-stimulated transfected CCD-18Co cells. h The mRNA expression of ICAM-1 in OSM-stimulated transfected CCD-18Co cells. i Western blot assay of ICAM-1 expression in OSM-stimulated transfected CCD-18Co cells. Data were presented as mean ± SEM of three independent experiments. *p < 0.05 and **p < 0.01, compared with OSM-stimulated CCD-18Co cells, were measured by one-way ANOVA.