Fig. 6: MLN4924 has therapeutic effect on the HFD-induced hepatic steatosis. | Cell Death & Disease

Fig. 6: MLN4924 has therapeutic effect on the HFD-induced hepatic steatosis.

From: Neddylation of sterol regulatory element-binding protein 1c is a potential therapeutic target for nonalcoholic fatty liver treatment

Fig. 6

a−h were fed the HFD for 8 weeks. After 8 weeks, the mice were divided into two groups, one fed HFD for 8 weeks and the other fed HFD and injected with MLN4924 (n = 6 per group). MLN4924 was injected intraperitoneally twice weekly for 8 weeks. a Schematic diagram of in vivo model. b Representative images showing the morphology, H&E staining, and Oil O Red staining of liver sections from HFD-fed mice (n = 6 images per group). c Mice were weighed for 16 weeks. d Liver weight was measured at the end of experiment (n = 6 per group). *P < 0.05 vs. control group. e ALT levels were measured in the serum of mice (n = 6 per group). f Triglyceride levels in the serum of mice (n = 6 per group). g Triglyceride concentrations in the liver tissue extracted from mice (n = 6 per group). h Immunohistochemical staining was applied to visualize the expression profile of SREBP1c in the liver of mice (n = 6 per group). i Expression level of SREBP1c in liver tissues of mice. The band intensities of SREBP1c protein were calculated using ImageJ and plotted by graph (mean ± SD, n = 6). j Total RNAs were extracted from liver tissues of mice. SREBP1c, ACC, FASN, and SCD1 mRNA levels were quantified by RT-qPCR. Results were quantified as the relative levels vs. 18S RNA level. All data were presented as the mean ± SD (n = 6 per group).

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