Fig. 2: The effects of miR-548t-5p on PC cells proliferation, migration, and invasion. | Cell Death & Disease

Fig. 2: The effects of miR-548t-5p on PC cells proliferation, migration, and invasion.

From: The YY1/miR-548t-5p/CXCL11 signaling axis regulates cell proliferation and metastasis in human pancreatic cancer

Fig. 2

a, b Relative expression of miR-548t-5p by qRT-PCR in PC cells with miR-548t-5p-mimics and inhibitor. c–g The colony formation assay (c) and EdU assay (d–g) were performed to analyze the effects of miR-548t-5p-mimics or inhibitor on cell proliferation. Magnification, ×100; scale bar, 100 μm in d, f. h, i The flow cytometry assay analyzed the effect of miR-548t-5p on cell cycle status. j, k Wound-healing assays measured the effect of miR-548t-5p on PC cell migration ability. l Transwell assays measured the effect of miR-548t-5p on PC cell invasion ability. m EMT-related proteins were analyzed by western blotting assays in transfected PC cells. The data are presented as mean ± SD from three independent experiments. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001.

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