Fig. 4: p57KIP2 was identified as potential target regulated by NSUN2 by RNA sequencing. | Cell Death & Disease

Fig. 4: p57KIP2 was identified as potential target regulated by NSUN2 by RNA sequencing.

From: RNA methyltransferase NSUN2 promotes gastric cancer cell proliferation by repressing p57Kip2 by an m5C-dependent manner

Fig. 4

a RNA was isolated from SGC 7901 and MGC 803 cells with stable knockdown of NSUN2 and p57Kip2 mRNAs expression was determined by qRT-PCR. Data were showed as the mean ± SD; *p < 0.05. b The protein levels of p57Kip2 and β-actin was assessed by western blot. c The mRNA half-life (t1/2) of p57Kip2 in SGC 7901 and MGC 803 cells with stable knockdown of NSUN2 or corresponding wild-type cells. d, e The relative mRNA and protein expression of p57Kip2 in NSUN2 knockdown cells or corresponding wild-type cells, transfected with small-interfering RNAs (siRNAs), was tested using qRT-PCR and western blot. siNC: negative control siRNA; sip57Kip2: siRNA against p57Kip2. Data were showed as the mean ± SD; *p < 0.05. f, g CCK-8 and colony formation assays were performed to determine the growth ability of NSUN2 knockdown cells after transfection of siRNA against p57Kip2. Data were showed as the mean ± SD; *p < 0.05.

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