Fig. 5: NSUN2 destabilized the p57Kip2 mRNA relies on its methyltransferase activity and m5C modifications in the 3′- UTR of p57Kip2 mRNA. | Cell Death & Disease

Fig. 5: NSUN2 destabilized the p57Kip2 mRNA relies on its methyltransferase activity and m5C modifications in the 3′- UTR of p57Kip2 mRNA.

From: RNA methyltransferase NSUN2 promotes gastric cancer cell proliferation by repressing p57Kip2 by an m5C-dependent manner

Fig. 5

a m5C dot blot assay of overexpress or knockdown of NSUN2 in MGC 803 and SGC 7901 cells, methylene blue staining (as control). b, c Pezx-FR02- p57Kip2 -3′-UTR plasmid with either wild-type or mutant (CCT mutation) m5C sites were constructed. The pattern diagram was shown. Above constructed plasmid was transfected into stable knockdown of NSUN2 or corresponding wild-type cells. Firefly luciferase activity was measured and normalized to Renilla luciferase activity. Data were presented as the mean ± SD; *p < 0.05. d m5C RIP and qRT-PCR analysis of m5C level in mRNA of p57Kip2 in MGC 803 cells transduced with stable knockdown of NSUN2 or corresponding wild-type cells. Data were showed as the mean ± SD; *p < 0.05.

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