Fig. 5: BAX and BAK are essential for S63845/venetoclax combination response through the BAX/BAK heterocomplex formation. | Cell Death & Disease

Fig. 5: BAX and BAK are essential for S63845/venetoclax combination response through the BAX/BAK heterocomplex formation.

From: Dual targeting of BCL2 and MCL1 rescues myeloma cells resistant to BCL2 and MCL1 inhibitors associated with the formation of BAX/BAK hetero-complexes

Fig. 5

a KMM1 and LP1 HMCLs were transfected with scramble, BAX and/or BAK siRNA for 48 h followed by 24 hours of S63845 and/or venetoclax. Results represent the mean ± SD of five independent experiments. Mann–Whitney test was used for statistical analysis (*p < 0.05, **p < 0.01). b HMCLs were treated with S63845 (25 nM for KMM1, 100 nM for LP1), venetoclax (100 nM) or a combination of both during 3 h. Immunoprecipitation reactions were performed overnight using the 6A7 BAX-Agarose coupled mAb. Immunoprecipitation eluates were analyzed by western blot. c CD138 purified primary cells were treated or not with the S63845 (25 nM)/venetoclax (100 nM) combination during 3 h and processed as in b.

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