Fig. 3: PD-L1 expression on endothelial cells affects the proliferation and activation of immune cells. | Cell Death & Disease

Fig. 3: PD-L1 expression on endothelial cells affects the proliferation and activation of immune cells.

From: anlotinib alters tumor immune microenvironment by downregulating PD-L1 expression on vascular endothelial cells

Fig. 3

a PBMC was extracted from normal blood and treated with 2.5 µg/ml anti-CD3 antibody and IL-2 (100 U/ml) for 72 h, and co-cultured with tumor-derived endothelial cells (Td-EC) for 48 h. b HUVECs were treated with the supernatant of tumor cells (CM) for 48 h. c PBMC and Td-EC were treated with control, anlotinib (0.1 μm) or anti-PD-L1 antibody (4 μg/ml). d Detection of the CD8+ IFN-γ+, CD8+Ki67+, CD8+GranzymeB+, and FoxP3+ T cells by flow cytometry. e Quantification of the above positive cells in the immune cells. f Quantification of cytokine levels in co-culture supernatants (2 days) by liquid microarray. Data are mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, Student’s t test.

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