Fig. 3: PDGFR-mediated PI3K signaling is required for VEGF or PDGF-induced CXCR7 expression. | Cell Death & Disease

Fig. 3: PDGFR-mediated PI3K signaling is required for VEGF or PDGF-induced CXCR7 expression.

From: Atypical chemokine receptor ACKR3/CXCR7 controls postnatal vasculogenesis and arterial specification by mesenchymal stem cells via Notch signaling

Fig. 3

The CXCR7 mRNA levels (a) and its cell surface levels (b) in ihMSCs pretreated with vehicle (DMSO), U-73122 (phospholipase C inhibitor, 10 μM), U0126 (MEK inhibitor, 20 μM), or LY294002 (PI3K inhibitor, 10 μM) for 30 min followed by stimulation with VEGF (12 ng/ml) for 24 h. Data are means ± SD (n = 9). *p < 0.01 compared with the control (untreated) group. The CXCR7 mRNA levels (c) and its cell surface levels (d) in ihMSCs pretreated for 30 min with vehicle (DMSO) or LY294002 (10 μM) followed by stimulation with PDGF-BB (10 ng/ml) for 24 h. e The percentage of CD31+ endothelial cells for ihMSCs treated with or without LY294002 followed by stimulation with PDGF-BB for 7 days. Data are means ± SD (n = 9). *p < 0.01 compared with the control (untreated) group. #p < 0.0001 compared with the vehicle group.

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