Fig. 2: HMG box is indispensable for SOX1 to promote NPC cell differentiation. | Cell Death & Disease

Fig. 2: HMG box is indispensable for SOX1 to promote NPC cell differentiation.

From: SOX1 promotes differentiation of nasopharyngeal carcinoma cells by activating retinoid metabolic pathway

Fig. 2

a Confocal immunofluorescence for SOX1 (red) and DAPI (blue) in HONE1 and CNE2 cells. Fluorescence images are respectively merged to display location of SOX1 (red). Scale bar = 4 µm. b Schematics of full length and mutated SOX1 proteins used in this study. c Morphology of HONETRE-(X) and CNE2TRE-(X) cells (X stand for vehicle, wild type SOX1 or mutant SOX1) under doxycycline treatment for 3 days. Scale bar = 50 µm. d Confocal immunofluorescence for SOX1 (red) and DAPI (blue) in HONETRE-(X) and CNE2TRE-(X) cells under doxycycline treatment for 3 days. Scale bar = 4 μm. e Western blot analysis of SOX1, KRT5, KRT13, and β-actin expression in HONETRE-(X) and CNE2TRE-(X) SOX1 cells under doxycycline treatment for 3 days. β-actin was used as a control. f SA-β gal staining (left panel) of HONETRE-(X) and CNE2TRE-(X) cells under doxycycline treatment for 7 days. Red arrows represent SA-β gal-positive cells. Scale bar = 50 μm. Dot plots (right panel) show quantification of the frequency of SA-β gal-positive cells in each vision. All data represent the mean ± SD (n = 5, ****P < 0.0001).

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