Fig. 3: Expression of circ5615 promoted cell proliferation in CRC cells.
From: circ5615 functions as a ceRNA to promote colorectal cancer progression by upregulating TNKS
a The interfering efficiencies of siRNA in SW480 cells were determined by RT-PCR. b Expression levels of circ5615 and NFATC3 in SW480 cells after transduction with circ5615 vector. c–e Circ5615 facilitated the proliferation of CRC cells shown by the RTCA (c), colony formation (d), and EdU (e) assays with silencing or overexpressing circ5615. Cell numbers were determined using the ImageJ program. f Cell cycle analysis of SW480 cells with silencing or overexpressing circ5615. Data are shown as mean ± SD (n = 3) or typical photographs of one representative experiment. Similar results were obtained in three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001; ns nonsignificant, paired t-test.
