Fig. 4: Identification of miR-149-5p binding to circ5615.
From: circ5615 functions as a ceRNA to promote colorectal cancer progression by upregulating TNKS
a Schematic flowchart illustrated the pipelines for miRNA analyses. b RTCA proliferation analysis for SW480 cells transfected with miR-149-5p mimics. c The Ago2 RIP showed that Ago2 served as a platform for circ5615, miR-149-5p and miR-331-3p. d Schematic illustration showed the potential binding sites of miR-149-5p and miR-331-3p on circ5615. e Luciferase activity of circ5615 transfected with miR-149-5p or miR-331-3p mimics. Firefly luciferase activity was normalized by Renila luciferase activity. f Biotin-coupled CircRNA pull-down assays confirmed that miR-149-5p was effectively enriched by circ5615 in SW480 cells. g, h circ5615 was enriched with 3′-end biotinylated miR-149-5p in SW480 and HCT 116 cells. i The FISH assays showed that circ5615 and miR-149-5p were relatively co-localized in the cytoplasm of SW480 cells. j Luciferase reporter activity of circ5615 co-transfected with miR-149-5p mimics or mimics NC. Data are shown as mean ± SD (n = 3). Similar results were obtained in three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001, paired t-test.
