Fig. 5: TNKS was identified as a direct target of miR-149-5p.
From: circ5615 functions as a ceRNA to promote colorectal cancer progression by upregulating TNKS
a Heat map analyses showed top 500 activated and suppressed genes by RNA-seq (left) and corresponding gene expression by microarray (right). SI: circ5615 knockdown. OE: circ5615 overexpression. b The GO biological processes analysis of the circ5615-regulated genes (fold changes >1.41 in RNA-seq samples and <0.80 in microarray samples; fold changes <0.70 in RNA-seq samples and >1.20 in microarray samples, p < 0.05). c Four target genes were showed with the overlap between circ5615-regulated genes and predicted miR-149-5p targets. d The expression of genes after circ5615 knockdown or overexpression in SW480 cells. e RT-PCR analysis revealed TNKS, LUC7L3 and SHROOM2 expression were significantly decreased by miR-149-5p in SW480 cells. f The protein levels of TNKS in the CRC cells transfected with miR-149-5p mimics. g Luciferase reporter activity of TNKS-3′UTR wild-type-reporter or mutant- reporter in HEK-293T cells transfected with miR-149-5p mimics or mimic NC. h Biotin-coupled miRNA pull-down assays determined that TNKS was effectively enriched by miR-149-5p. Data are shown as mean ± SD (n = 3). Similar results were obtained in three independent experiments or typical photographs of one representative experiment. *p < 0.05; **p < 0.01; ***p < 0.001; ns nonsignificant, paired t-test.
