Fig. 6: circ5615 promoted cell proliferation by relieving the repression of miR-149-5p for TNKS expression.
From: circ5615 functions as a ceRNA to promote colorectal cancer progression by upregulating TNKS
a The protein levels of TNKS, AXIN2, β-catenin, and CCND1 in the CRC cells with knockdown of circ5615. SI: circ5615 knockdown. Fibrillarin served as a specific nuclear marker. b Positive correlation between the levels of circ5615 and TNKS of the 35 CRC tumorous tissues. ΔCt values were normalized according to ACTB. c Luciferase reporter activity of TNKS-3′UTR wild-type-reporter or mutant-reporter in HEK-293T cells with the knockdown or overexpression of circ5615. d Cellular expression of TNKS and β-catenin in HCT 116 cells after circ5615 knockdown. Nuclei was stained with DAPI (blue). e The mRNA (upper panel) and protein (lower panel) levels of TNKS expression in SW480 cells transfected with miR-149-5p mimics alone or co-transfected with circ5615. f RTCA cell proliferation analysis in SW480 cells transfected with miR-149-5p mimics alone or co-transfected with circ5615. g Upper panel: the TNKS mRNA expression in SW480 cells with circ5615 overexpression and TNKS knockdown. Lower panel: the protein levels of TNKS, AXIN2, β-catenin, and CCND1 in SW480 cells with circ5615 overexpression and TNKS knockdown. Fibrillarin served as a specific nuclear marker. h RTCA cell proliferation assays in SW480 cells with circ5615 overexpression after TNKS knockdown. i Cell cycle analysis of SW480 cells with circ5615 overexpression after TNKS knockdown. Data are shown as mean ± SD (n = 3) or typical photographs of one representative experiment. *p < 0.05; **p < 0.01; ***p < 0.001; ns nonsignificant, paired t-test.
