Fig. 2: HDAC7 promotes NPC cell proliferation, migration, and invasion in vitro and growth in vitro.
From: HDAC7 promotes the oncogenicity of nasopharyngeal carcinoma cells by miR-4465-EphA2 signaling axis
a Establishment of HK1 and 5–8F cell lines with stable knockdown of HDAC7 by shRNA (shHDAC7) and their control cell lines (shNC). b–f HDAC7 knockdown inhibits NPC cell proliferation, migration and invasion in vitro. CCK-8 (b), plate clone formation (c), and EdU incorporation (d) assay showing the proliferation of HK1 and 5–8F cells with shHDAC7 and their control cells. e Scratch wound healing showing the migration of HK1 and 5–8F cells with shHDAC7 and their control cells. f Transwell Matrigel invasion assay showing the invasion of HK1 and 5–8F cells with shHDAC7 and their control cells. g Xenograft growth of HK1 and 5–8F cells with shHDAC7 and their control cells. (Top) The photography of xenograft tumors after 20 days subcutaneous implantation of the cells; (bottom) growth and weight of the xenograft tumors. n = 5 mice per group. Means, SDs, and statistical significance are denoted; *P < 0.05; **P < 0.01; ***P< 0.001; ns no significance.
