Fig. 2: Functional roles of CANT1 lncRNA in RB. | Cell Death & Disease

Fig. 2: Functional roles of CANT1 lncRNA in RB.

From: LncRNA CANT1 suppresses retinoblastoma progression by repellinghistone methyltransferase in PI3Kγ promoter

Fig. 2

a, b A CCK8 assay was performed to measure the cell growth rate of CANT1-overexpressing Y79 and Weri-Rb1 cells. The experiments were performed in triplicate, and the absorbance at day 1 was set as 100%. *P < 0.05 compared with the control and mock. c, d A soft agar assay was used to assess the tumor formation ability in vitro. c Small colonies were observed and counted under the microscope. d Colony count statistics showed a significant reduction in colonies formed by CANT1-overexpressing cells. Colony numbers were counted in three independent soft agar plates. All of the data are presented as the mean ± SEM. *P < 0.05 compared with the control and mock. Scale bar: 600 μm. e, f Eye weight of the orthotropic xenograft formed by Weri-Rb1 cells injected into the vitreous cavity with or without CANT1 overexpression at 40 days after implantation; n = 7, *P < 0.05 compared with the mock. f Representative images of H&E staining for the evaluation of tumor formation in vivo. Scale bar: 200 μm.

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