Fig. 3: Regulatory targets of CANT1 lncRNA in RB. | Cell Death & Disease

Fig. 3: Regulatory targets of CANT1 lncRNA in RB.

From: LncRNA CANT1 suppresses retinoblastoma progression by repellinghistone methyltransferase in PI3Kγ promoter

Fig. 3

a The overlapping genes presenting differential expression between the two RB cell lines are shown. Twelve genes were co-upregulated, and 454 genes were co-downregulated (fold change ≥ 1.5, FDR < 0.05). b KEGG analysis for all genes with differential expression. c Heatmap of genes in the PI3K/Akt signaling pathway. d Real-time PCR was performed to measure the PI3Kγ mRNA level in tumor and normal cells. All of the data are presented as the mean ± SEM. *P < 0.05: compared with the control and mock. e Western blot showing the protein levels of PI3Kγ, p-AKT, and pan-AKT in tumor and normal cells. GAPDH was used as internal control. f Immunohistochemical staining of PI3Kγ in RB and normal tissues. PI3Kγ expression in tumor sections was higher than normal tissues. Scale bar: 200 μm. g Two siRNAs were used to silence PI3Kγ, as examined by Western blot.

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