Fig. 4: Oncogenic role of PI3Kγ in RB.

a, b CCK8 assay showed that the cell growth rate decreased after the silencing of PI3Kγ. All of the data are presented as the mean ± SEM. *P < 0.05: compared with the control and NC. c Colony formation assay was used to assess the cell growth rate of tumor cells treated with siRNA. d Small colonies on each plate were counted. All of the data are presented as the mean ± SEM. *P < 0.05: compared with control. e A soft agar assay was performed to estimate the in vitro tumor formation ability of Y79 and Weri-Rb1 cells treated with siRNA. f Quantification of visible colonies on each plate. All of the experiments were performed in triplicate, and the number of colonies formed is shown as the mean ± SEM. *P < 0.05.