Fig. 6: CANT1 modulates PI3Kγ transcription by abolishing H3K4 methylation. | Cell Death & Disease

Fig. 6: CANT1 modulates PI3Kγ transcription by abolishing H3K4 methylation.

From: LncRNA CANT1 suppresses retinoblastoma progression by repellinghistone methyltransferase in PI3Kγ promoter

Fig. 6

a Schematic diagram of the PI3Kγ and the GAPDH promoter regions. Arrow: transcriptional direction; sites X–Z: different sites used in this assay. b–d PCR examination of histone H3K4 trimethylation changes in the PI3Kγ promoter (c) and GAPDH promoter (d) upon CANT1 overexpression in Y79 and Weri-Rb1 cells. IgG was used as a negative control. Input: total RNA was reverse transcribed before incubation and amplified with primers; M: marker. e, f Real-time qPCR examination of histone H3K4 trimethylation changes in the PI3Kγ promoter. Site X is 6 kb upstream of the PI3Kγ TSS. All data are presented as the means ± SEM. *P < 0.05: compared with the control and mock.

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