Fig. 2: circMAST1 locates in cytoplasm. | Cell Death & Disease

Fig. 2: circMAST1 locates in cytoplasm.

From: The circular RNA circMAST1 promotes hepatocellular carcinoma cell proliferation and migration by sponging miR-1299 and regulating CTNND1 expression

Fig. 2

a circMAST1 in HCC adjacent non-tumor and tumor tissues was detected by FISH. b The expression levels of circMAST1 in multiple HCC cell lines (*P < 0.05; ***P < 0.001; n = 4). c Illustration showing the siRNA targeting the back-splice junction (si-circMAST-1, si-circMAST1-2 and si-circMAST1-3); qRT-PCR results for circMAST1 in HCLLM3 and hepG2 cells treated with or without siRNA (si-NC, control oligonucleotides with scramble sequence; si-circMAST-1, si-circMAST1-2 and si-circMAST1-3, oligonucleotides targeting the back-splice junction) (**P < 0.01; ***P < 0.001; n = 4). d, e FISH confirmed that circMAST1 was predominantly located in cytoplasm. Nuclei were stained with DAPI. u6, 18s and circMAST1 were labeled with cy3.

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