Fig. 5: Loss of HMGB1 in hepatocytes correlates with reduced proliferation in the tumor.
From: The HMGB1-RAGE axis modulates the growth of autophagy-deficient hepatic tumors
a, b Liver sections from 15-month-old mice of different genotypes were immunostained with anti-PCNA (a), or anti-Cyclin D (b). White arrow indicated proliferating hepatocytes. White dotted lines indicate the tumor border. c Immunoblot analysis of PCNA, cyclin D1, and cyclin E proteins in the tumor or non-tumor sample of 15-month-old Atg7ΔHep and, Atg7/Hmgb1ΔHep mice. d Densitometry qualification of the indicated proteins. e The hepatic mRNA level of indicated genes were determined in the indicated tissues of 15-month-old mice of different genotypes, determined by real-time PCR. NT, non-tumor, T, tumor. Data are reported as mean ± SE, *P < 0.05, **P < 0.01, n.s., no significance; n = 3 mice per group.
