Fig. 2: SOX2OT upregulation confers TMZ resistance, facilitates cell proliferation, and inhibits cell apoptosis in GBM cells. | Cell Death & Disease

Fig. 2: SOX2OT upregulation confers TMZ resistance, facilitates cell proliferation, and inhibits cell apoptosis in GBM cells.

From: LncRNA SOX2OT promotes temozolomide resistance by elevating SOX2 expression via ALKBH5-mediated epigenetic regulation in glioblastoma

Fig. 2

a The TMZ sensitivity of U87TR and U251TR cells after sh-NC or sh-SOX2OT transfection measured by CCK-8 assay. *p < 0.05 compared with sh-NC group cells. b Cell viability of U87TR and U251TR sh-NC or sh-SOX2OT cells treated with TMZ (50 μg/ml) for 24, 48, 72, and 96 h. *p < 0.05 compared with sh-NC group cells. c The TMZ sensitivity of U87 and U251 cells after LV-NC or LV-SOX2OT transfection measured by CCK-8 assay. *p < 0.05 compared with LV-NC group cells. d Cell viability of U87 and U251 LV-NC or LV-SOX2OT cells treated TMZ (50 μg/ml) for 24, 48, 72, and 96 h. *p < 0.05 compared with LV-NC group cells. e–g The rate of apoptosis of sh-SOX2OT or LV-SOX2OT cells treated with TMZ (50 μg/ml) for 48 h was determined by the Annexin-V/FITC double staining. *p < 0.05 compared with sh-NC or LV-NC group cells. h–k The rate of EdU cells in sh-SOX2OT or LV-SOX2OT cells treated with TMZ (50 μg/ml) for 48 h was determined by EdU staining. *p < 0.05 compared with sh-NC or LV-NC group cells. Scar bar = 100 μm. Data are presented as mean ± S.D. of three independent experiments.

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