Fig. 3: cGAS–STING pathway is activated in irradiated MSCs. | Cell Death & Disease

Fig. 3: cGAS–STING pathway is activated in irradiated MSCs.

From: Irradiation induces cancer lung metastasis through activation of the cGAS–STING–CCL5 pathway in mesenchymal stromal cells

Fig. 3

a MSCs were subjected to immunofluorescence staining with γ-H2AX antibody at 2 h after irradiation (12 Gy); scale bar, 25 µm. b MSCs were irradiated (12 Gy) for 48 h, staining with DAPI. Nuclei and micronuclei are shown; scale bar, 25 µm. c, d The expression levels of cGAS(C) and STING(D) were detected by immunofluorescence; scale bar (c), 75 µm; scale bar (d), 75 µm. e MSCs were irradiated (12 Gy) and cultured for 24 h, and then the expression level of ISGs were determined by real-time PCR. All experiments in this figure were repeated at least three times. *p < 0.05, **p < 0.01, and ****p < 0.0001.

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