Fig. 2: YBX1 promotes autophagy in NSCLC cells.
From: YBX1 mediates autophagy by targeting p110β and decreasing the sensitivity to cisplatin in NSCLC
H1299 and H460 cells were transfected with YBX1-specific siRNA, A549, and Hcc827 cells were transfected with YBX1-overexpression plasmids for 48 h. a YBX1 expression in H1299 and H460 cells were transfected with YBX1-specific siRNA or in A549 and Hcc827 cells were transfected with YBX1-overexpression plasmids was analyzed by western blot. b LC3I/II expression in H1299 and H460 cells were transfected with YBX1-specific siRNA or in A549 and Hcc827 cells were transfected with YBX1-overexpression plasmids was analyzed by western blot, (c) immunofluorescence, and (d) TEM-based ultrastructure analysis (autophagosomes). Data represent the mean ± SD of three independent experiments. *P < 0.05 and **P < 0.001 vs. control. e GFP-LC3 was co-transfected with YBX1 siRNA in H1299 and H460 cells, then treated with rapamycin at 471 nmol/L and 200 nmol/L, respectively, and co-transfected with YBX1-overexpressing plasmids in A549 and HCC827 cells, treated with 3BDO at 24 µmol/L and 120 µmol/L, respectively. The punctate GFP-LC3 was detected by immunofluorescence, and (f) the GFP-LC3 or GFP-LC3II conversation was detected by western blot. Data represent the mean ± SD of three experiments, *P < 0.05, **P < 0.01 vs. control.
