Fig. 1: LncRNA SNORD3A is downregulated in breast cancer. | Cell Death & Disease

Fig. 1: LncRNA SNORD3A is downregulated in breast cancer.

From: LncRNA SNORD3A specifically sensitizes breast cancer cells to 5-FU by sponging miR-185-5p to enhance UMPS expression

Fig. 1

a SNORD3A level was measured by qRT-PCR in a series of breast cancer cell lines. b SNORD3A level in paired breast cancer tissues and benign adjacent tissues was detected by qRT-PCR (n = 26). c Schematic of the coding potential and ribosome-binding sites of the SNORD3A transcript predicted by online analysis. d, e The full transcript of SNORD3A or potential ORF was cloned into the pEGFP-N1 vector with a mutation in the initiation codon of GFP and HEK293T cells were transfected as indicated. GFP fluorescence was observed (d) and GFP fusion protein levels were examined by western blot using anti-GFP antibody (e). Scale bar, 100 μm. f Fractionation of MCF10A, MDA-MB-231/SNORD3A, and MCF-7/SNORD3A cells followed by qRT-PCR. BCAR4 served as a positive control for nuclear fractions, and GAPDH functioned as a control for cytoplasmic fractions. Student’s t test, mean ± s.d. ****p < 0.0001.

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