Fig. 6: Effects of CUEDC2 on osteoclast differentiation.
From: CUEDC2 controls osteoblast differentiation and bone formation via SOCS3–STAT3 pathway
a–c Bone marrow macrophages were infected with pMX-IRES-EGFP (pMX-GFP) or CUEDC2 retrovirus, and the cells were then cultured with M-CSF (30 ng/ml) and RANKL (100 ng/ml) for 3 days. a Total RNA was extracted from the cultured cells, and then real-time PCR was performed using CUEDC2-, NFATc1-, and TRAP-specific primers. **P < 0.01, ***P < 0.001 versus pMX-GFP. b The cells were stained for TRAP. c The number of TRAP-positive multinucleated cells (MNCs) per well was counted. d–f BMMs were transfected with siRNA control or siRNA CUEDC2 (25 nM). After 24 h, cells were cultured for 3 days in the presence of M-CSF (30 ng/ml) and RANKL (100 ng/ml). d Real-time PCR analysis. *P < 0.05 versus si-control. e TRAP staining. f The number of TRAP-positive multinucleated cells.
