Fig. 3: The effect of bone-targeted OGRU on the function of osteoblasts in HLU mice.

a Representative images of immunofluorescence staining and H&E staining of same sections in the femurs of mice. The pcDNA3.1(+)–OGRU was labeled with GFP (green, left column). Immunofluorescence staining of Ocn was performed to detect osteoblasts (red, middle-left column). Merged images with DAPI staining showed co-staining of pcDNA3.1(+)–OGRU and osteoblasts. H&E staining was included in the right column. Scale bar = 20 μm (n = 5). b Schematic of the experimental design. c Representative images of osteocalcin immunohistochemical staining in the femurs of mice in the indicated groups (n = 5). Scale bars: 50 μm. d Representative images of Goldner’s trichrome staining of the trabecular bone at distal femurs of mice in the indicated groups (n = 5). Osteoid stains are shown in red (indicated by the black arrows), and mineralized bone stains in green. Scale bars: 50 µm. e Representative images of calcein double labeling (n = 5). Scale bars: 10 μm. All data are the mean ± SD. *P < 0.05, **P < 0.01. NS not significant.