Fig. 3: HJURP promotes PDAC growth in vitro and in vivo. | Cell Death & Disease

Fig. 3: HJURP promotes PDAC growth in vitro and in vivo.

From: Holliday junction recognition protein promotes pancreatic cancer growth and metastasis via modulation of the MDM2/p53 signaling

Fig. 3

a, b MTT assays in HJURP overexpression (a) and knockdown (b) PDAC cells. Capan-2 (a) and SW 1990 (b) cells were transfected with HJURP or shHJURP vectors, respectively. c–f Pancreatosphere formation (c, e) and its quantification (d, f) of PDAC cells mentioned in a and b. g–l Representative ventral view images (g, j) and its quantification (h, k) of bioluminescence from xenograft mice transplanted with cells described above. The representative images, IHC analysis of Ki-67-positive cells of mouse allograft tumors from Capan-2 (i) and SW 1990 (l; n = 5). All experiments were repeated at least three times, and representative data are shown. Scale bars, 100 μm (c, e) and 20 μm (i, l); data are means ± SEM; **p < 0.01.

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