Fig. 1: LPS increased TMEM16A expression. | Cell Death & Disease

Fig. 1: LPS increased TMEM16A expression.

From: Dual role of Ca2+-activated Cl− channel transmembrane member 16A in lipopolysaccharide-induced intestinal epithelial barrier dysfunction in vitro

Fig. 1

a, b Expression analysis of TMEM16A by qRT-PCR and Western blotting in IEC-6 cells incubated with LPS. (n = 3/group). c Expression analysis of TMEM16A by Western blotting in RAW264.7 cells incubated with LPS. (n = 3/group) d, e Effects of siRNA-targeting NF-κB p65 on TMEM16A expression in IEC-6 cells incubated with LPS studied by Western blotting and confocal microscope (green fluorescence), scale bar = 20 μm. (n = 6/group). Data are expressed as the mean ± SD. Values in the normal control (NC) group are set to 100% and other values are given relative to those in the NC group, *p < 0.05, **p < 0.01 compared with NC group. si-NF-κB p65, in the presence of siRNA-targeting NF-κB p65.

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