Fig. 1: Effects of lithium pilocarpine in the pGFAP-BDNF mice. | Cell Death & Disease

Fig. 1: Effects of lithium pilocarpine in the pGFAP-BDNF mice.

From: Astrocytic BDNF and TrkB regulate severity and neuronal activity in mouse models of temporal lobe epilepsy

Fig. 1

a Schematic representation of the experimental design. b Representative picture showing WT (left) and pGFAP-BDNF (right) mice 10 days after pilocarpine (Pilo) treatment. c All the items of the SHIRPA battery test are pulled as a mean composite score. Statistical analysis indicate that pilocarpine induces a significant increase in abnormal scores (two-way ANOVA, variable treatment: F(3,29) = 79.71; p < 0.001) and that pGFAP-BDNF mice exhibit the worse score (post hoc test, p < 0.001). d Pathlength in a 30-min open-field session is depicted. Results indicate strong significant differences between groups (two-way ANOVA, variable treatment: F(3,27) = 19.81, p < 0.001). WT + Pilo mice displayed a significant increase on locomotor activity compared to WT + Veh (post hoc test: p < 0.001) whereas pGFAP-BDNF + Pilo mice displayed a significant decrease on locomotor activity compared to pGFAP-BDNF + Veh mice (post hoc test: p < 0.001). e Fluoro-Jade staining was performed 10 days after pilocarpine treatment. Photomicrographs show the hippocampal areas positive for Fluoro-Jade in WT (left image) and pGFAP-BDNF (right image) mice treated with pilocarpine. Scale bar, 500 μm. f Graph showing the quantification of the Fluoro-Jade-positive cells per field in CA1, CA3, and CA4. Statistical analysis indicated that pGFAP-BDNF mice showed significant higher rates of neuronal cell death than WT mice when treated with pilocarpine (two-way ANOVA, variable genotype: F(1,27) = 26.42, p < 0.001). Bars represent mean ± SEM. Data were analyzed by two-way analysis of variance (ANOVA) with Bonferroni’s test as a post hoc. *p < 0.05 and ***p < 0.001 when compared with vehicle controls; $p < 0.05 and $$p < 0.01 when compared with WT + Pilo. For behavioral experiments: WT + Veh (n = 9), WT + Pilo (n = 6), pGFAP-BDNF + Veh (n = 9), and pGFAP-BDNF + Pilo (n = 7). For biochemical experiments: WT + Veh (n = 5), WT + Pilo (n = 7), pGFAP-BDNF + Veh (n = 6), and pGFAP-BDNF + Pilo (n = 8). For the Fluoro-Jade experiment: WT + Pilo (n = 5) and pGFAP-BDNF + Pilo (n = 6). All mice were used at 10 weeks of age and were males.

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