Fig. 5: Effects of specific genetic deletion of TrkB in astrocytes or in neurons in the hippocampus of mice treated with lithium pilocarpine. | Cell Death & Disease

Fig. 5: Effects of specific genetic deletion of TrkB in astrocytes or in neurons in the hippocampus of mice treated with lithium pilocarpine.

From: Astrocytic BDNF and TrkB regulate severity and neuronal activity in mouse models of temporal lobe epilepsy

Fig. 5

Surgery was performed in mice in order to inject a specific AAV and after 3 weeks status epilepticus (SE) was induced using the lithium-pilocarpine model. For this, 24 h before pilocarpine application, lithium was administered. Thirty minutes before pilocarpine administration, the animals received scopolamine. Two hours after pilocarpine, the SE was stopped by using valium. Ten days after these procedures, the animals were sacrificed and analyzed (a). AAV-injection site in the mouse hippocampus: CA1 and DG (b). Representative images showing how CA1 and DG look after AAV injection are depicted (c). SO stratum orients, SP stratum pyramidale, SR stratum radiatum, SL-M stratum lacunosum moleculare, SM stratum moleculare, SG stratum granulare, H hilus. Scale bar: 200 μm. Representative immunoblots showing the levels of TrkB.FL and TrkB.T1 (d–e). The histograms represent the protein expression expressed as percentage of TrkBf/f-GFP-Veh. All data are shown as the mean ± SEM (n = 15 TrkBf/f-GFP-Veh; 10 TrkBf/f-GFP-Pilo; 8 TrkBf/f-GFAP-Cre-Pilo; 12 TrkBf/f-CaMKII-Cre-Pilo). Data were analyzed by one-way ANOVA followed by Tukey’s test. *p < 0.05, ***p < 0.001 compared to TrkBf/f-GFP-Veh. $$$p < 0.001 compared to TrkBf/f-GFP-Pilo. #p < 0.001 compared with TrkBf/f-CaMKII-Cre-Pilo.

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