Fig. 8: Circ ZNF566 promotes the growth and metastasis of HCC cells in vivo. | Cell Death & Disease

Fig. 8: Circ ZNF566 promotes the growth and metastasis of HCC cells in vivo.

From: Circular RNA circZNF566 promotes hepatocellular carcinoma progression by sponging miR-4738-3p and regulating TDO2 expression

Fig. 8

a Images of subcutaneous xenograft tumors derived from Huh7 and LM3 cells. b, c Tumor weights of Huh7 and LM3 cells are shown, and the tumor volumes of Huh7 and LM3 cells were measured seven times every 3 days. d, e TDO2 and Ki67 expression in subcutaneous xenograft tumors of Huh7 and LM3 cells was detected by IHC. f, g The protein expression levels of TDO2, Bcl-2, Ki67, Caspase, N-cadherin, E-cadherin, and Vimentin were detected by WB in subcutaneous xenograft tumors of Huh7 and LM3 cells. h, i Images of liver metastasis assays indicating that overexpression or knockdown of circZNF566 could increase or decrease the number of liver metastasis nodules compared with the NC, respectively. H&E staining and IHC revealed liver tumor metastasis. All data are from three independent experiments and are presented as the means ± SEM or representative of three independent experiments with similar results (*p < 0.05, **p < 0.01, ***p < 0.001).

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