Fig. 4: Circ-0039411 knockdown impeded cell proliferation, migration, EMT as well as tumor growth and metastasis in LUAD. | Cell Death & Disease

Fig. 4: Circ-0039411 knockdown impeded cell proliferation, migration, EMT as well as tumor growth and metastasis in LUAD.

From: Circ-MMP2 (circ-0039411) induced by FOXM1 promotes the proliferation and migration of lung adenocarcinoma cells in vitro and in vivo

Fig. 4

a, b EdU assay (scale bar = 100 μm) and colony formation experiment were conducted to estimate cell proliferation. c Cell migration ability was estimated by transwell assay (scale bar = 100 μm). d Western blot assay were carried out for testing EMT. GAPDH was an internal control. e Tumor volume in mice was detected after circ-0039411 was knocked down. f After circ-0039411 knockdown, tumor volume and weight of xenografts was tested. g IHC was carried out to detect proliferation and EMT indexes in xenografts after circ-0039411 knockdown (scale bar = 50 μm). h Number of metastatic nodules was detected by H&E staining (scale bar = 50 μm). i Western blots of EMT-related proteins in xenografts with circ-0039411 knockdown. **P < 0.01.

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