Fig. 6: Circ-0039411 enhanced the stability of FOXM1 mRNA via recruiting IGF2BP3. | Cell Death & Disease

Fig. 6: Circ-0039411 enhanced the stability of FOXM1 mRNA via recruiting IGF2BP3.

From: Circ-MMP2 (circ-0039411) induced by FOXM1 promotes the proliferation and migration of lung adenocarcinoma cells in vitro and in vivo

Fig. 6

a Subcellular fractionation was utilized to test the location of circ-0039411 in A549 and HCC827 cells. b RIP assay plus qRT-PCR analysis of circ-0039411 enrichment by anti-Ago2 in A549 and HCC827 cells. c StarBase was adopted to screen out RBPs which could combine with circ-0039411 and FOXM1. d qRT-PCR of circ-0039411 enrichment precipitated by 22 candidate RBPs via RIP assays. e Immunoblot following RNA pull down assay detected the level of IGF2BP3 in the pulldown of different groups. f RIP assay revealed the influence of circ-0039411 silence on the binding of IGF2BP3 to FOXM1 in LUAD cells. g The qRT-PCR and western blot confirmed the efficiency of silencing IGF2BP3. h, i qRT-PCR data of FOXM1 expression at 0, 4, and 8 h after Act D treatment under IGF2BP3 or circ-0039411 silencing. j qRT-PCR data and western blots of FOXM1 level under IGF2BP3 knockdown. GAPDH was the internal control. **P < 0.01.

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