Fig. 3: Influence of regulating miR-450b-5p and CRYAB in vivo.
From: Inhibition of miR-450b-5p ameliorates hepatic ischemia/reperfusion injury via targeting CRYAB
a–c Serum levels of IL-6, IL-1β, and TNF-α within 1-h ischemia followed by 1-, 3-, 6-, and 9-h reperfusion. d Expression of miR-450b-5p in the liver within 1-h ischemia followed by 1-, 3-, 6-, and 9-h reperfusion. e Hepatic pathologic alteration after inhibition of miR-450b-5p or CRYAB within 1-h ischemia followed by 6-h reperfusion, scale bar = 200 μm. f Hepatic apoptosis detected by TUNEL within 1-h ischemia followed by 6-h reperfusion, scale bar = 200 μm. g, h Levels of IL-6, IL-1β, TNF-α, AST, and ALT in mouse serum within 1-h ischemia followed by 6-h reperfusion. CRYAB shRNA-, antagomir-, and IRI group was settled as sham group. n = 5 for each group, *P < 0.05, **P < 0.01, ***P < 0.001, nsP > 0.05.
