Fig. 1: BIK protein levels in primary breast tumors and established cell lines. | Cell Death & Disease

Fig. 1: BIK protein levels in primary breast tumors and established cell lines.

From: BIK drives an aggressive breast cancer phenotype through sublethal apoptosis and predicts poor prognosis of ER-positive breast cancer

Fig. 1

a Western blot and quantitation (numbers at the bottom) of 16 primary breast tumor lysates probed with an anti-BIK antibody. PC indicates positive control—lysates prepared from HeLa cells treated with proteasome inhibitor MG132 known to upregulate BIK protein. Asterisk indicates nonspecific immunoreactive bands. b Anti-BIK western blot analysis of cell lysates prepared from five common breast cancer cell lines. Lane indicating PC—lysates prepared from MCF-7 cells transiently transfected with BIK-expressing plasmid were used as a positive control. c Western blot analysis of BIK expression in MCF-7 Tet-on cells using the indicated amounts of Dox stimulation for 24 h. d Western blot analysis of BIK expression in MDA-MB-231 Tet-on cells using indicated amounts of Dox stimulation for 24 h.

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