Fig. 4: Clonogenic survival assay of MCF-7 cells induced to express BIK.

a Top: Representative images of clonogenic survival assay performed for Empty vector or BIK-expressing MCF-7 Tet-on cells on continuous Dox stimulation at the indicated Dox concentrations over 11 days. Bottom: Bar graph depicting % clonogenic survival relative to untreated. One-way ANOVA followed by Sidak′s post-hoc test was performed to compute significance among groups. b Top: Representative images of colonies formed by MCF-7 Tet-on Empty vector or BIK-expressing cells at 250 ng/ml Dox stimulation. Arrows show colonies with frail morphology. Scale bar 1 mm. Bottom: Colony area was calculated for at least 350 colonies from each group from three different experiments. Error bars represent SEM. One-way ANOVA followed by Sidak′s post-hoc test was performed to compute significance among groups. c Left: Representative images depicting cellular density of colonies formed by MCF-7 Tet-on Empty vector or BIK-expressing cells. Red areas indicate high density whereas blue areas indicate low density. Right: Bar graph depicting quantitation of colony density. At least 350 colonies were analyzed from three independent experiments. Error bars indicate SEM. One-way ANOVA followed by Sidak′s post-hoc test was performed to compute significance among groups.