Fig. 1: POU4F1 was highly expressed in melanoma.

a POU4F1 mRNA level was analyzed by qRT-PCR in NHMs, melanocyte cell line (PIG1) and melanoma cell lines, n = 3. b POU4F1 protein expression was analyzed by immunoblotting in NHMs, melanocyte cell line and melanoma cell lines. c POU4F1 mRNA level was analyzed by qRT-PCR in nevus (N) and melanoma (M) tissues, n = 3. d Statistics analysis on the mRNA expression level of POU4F1 in nevus and melanoma tissues. e POU4F1 protein expression was analyzed by immunoblotting in nevus (N) and melanoma (M) tissues. f Densitometry analysis on the protein level of POU4F1 in nevus and melanoma tissues. Data are mean ± SEM, *p < 0.05, **p < 0.01.